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| MSPIRE International Research Experience |
Feb. 21 - Mar 25, 2011 |
Kyle Ann Lunsford, a graduate student in Richard Yost’s lab at the University of Florida, traveled to the FOM Institute AMOLF for a 5-week visit to Dr. Rob Heeren’s Bimolecular Imaging Mass Spectrometry Laboratory. Her research at UF has developed MALDI linear ion trap tandem MS imaging methods for the analysis of lignocellulosic materials (LCMs). Kyle’s time in Dr. Heeren’s lab focused on generating complementary data using ToF-SIMS and MALDI ion mobility to compare with the MALDI MS images of Populus stem tissue generated in Dr. Yost’s lab.
The images obtained from the ToF-SIMS offered the high spatial resolution needed to distinguish between different regions of tissue types, specifically between live and dead cells. In addition, these high spatial resolution images were also compared with fluorescence microscopy—the fluorescence images generated by the cellulose-selective fluorophore calcoflour white and the autofluorescence images of lignin were used to aid in ion identification of the ToF-SIMS images.
Outside the laboratory, Kyle was exposed to the rich Dutch culture, which included exploring museums such as the Rijksmuseum (pictured below), Van Gogh museum and the Anne Frank House. In addition, she was able to travel to the Dutch countryside to observe the windmills of Kinderdijk, which the first ones were built in the mid 1700s to control the water level of the polders.
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